Journal: Nanoscale Research Letters

Article Title: A nerve guidance conduit with topographical and biochemical cues: potential application using human neural stem cells

doi: 10.1186/s11671-015-0972-6

Figure Lengend Snippet: Fluorescence microscopy images of hNSCs cultured with PCL conduit for 14 days: a DAPI and b β-III tubulin. DAPI staining is indicated in blue and β-III tubulin staining is indicated in green. Arrow indicates the direction of aligned nanofibers

Article Snippet: After cryosectioning (20-μm slices), each section was washed twice in PBS for 10 min. Then, the samples were treated with blocking buffer (3 % goat serum PBS with 0.01 % Triton X-100) for 1 h at room temperature before adding β-III tubulin goat anti-mouse primary antibody (1:200 dilution, Promega, Madison, WI, USA) overnight at 4 °C.

Techniques: Fluorescence, Microscopy, Cell Culture, Staining

Journal: Nanoscale Research Letters

Article Title: A nerve guidance conduit with topographical and biochemical cues: potential application using human neural stem cells

doi: 10.1186/s11671-015-0972-6

Figure Lengend Snippet: Confocal microscopy images of hNSCs cultured with PSHU-RGD/PCL conduit for 14 days: representative images a , b along the center and e , f inner wall of microchannels, a , d DAPI, b , e β-III tubulin, and c , f combined DAPI and β-III tubulin images. DAPI staining is indicated in blue and β-III tubulin staining is indicated in green . Images show that hNSCs migrated far into the conduit with extensive neurite extension along the nanofiber direction ( arrows )

Article Snippet: After cryosectioning (20-μm slices), each section was washed twice in PBS for 10 min. Then, the samples were treated with blocking buffer (3 % goat serum PBS with 0.01 % Triton X-100) for 1 h at room temperature before adding β-III tubulin goat anti-mouse primary antibody (1:200 dilution, Promega, Madison, WI, USA) overnight at 4 °C.

Techniques: Confocal Microscopy, Cell Culture, Staining

Journal: Biochemistry and Biophysics Reports

Article Title: An appropriate loading control for western blot analysis in animal models of myocardial ischemic infarction

doi: 10.1016/j.bbrep.2017.09.001

Figure Lengend Snippet: Protein levels of β-tubulin, β-actin and GAPDH in the heart tissue from animal model of myocardial ischemia. (A, B) Western blot and quantitative analysis of β-tubulin, β-actin and GAPDH protein levels in heart tissue from Rhesus monkey model of early stages of myocardial ischemia (2 h after surgery, n = 2; 24 h after surgery, n = 2). (C, D) Western blot and quantitative analysis of β-tubulin, β-actin and GAPDH protein levels in heart tissue from Rhesus monkey model of myocardial infarction (4 months after surgery, n = 4). (E, F) Western blot and quantitative analysis of β-tubulin, β-actin and GAPDH protein levels in heart tissue from mouse model of different time point (1 d after surgery, n = 6; 4 d after surgery, n = 6; 7 d after surgery, n = 6). Sham: sham operated control (n = 8); IA: ischemic/ infarct area; RA: remote area. Blots were analyzed by densitometry using Fusion-Capt Software. Results are presented as means ± SD. Three independent experiments were run for each result. *: P < 0.05 versus sham operated control; #: P < 0.05.

Article Snippet: The blots were then incubated with primary antibodies anti-β-actin, GAPDH, and β-tubulin, (at 1:1000 dilution, ZSGB-BIO, China) overnight at 4 °C.

Techniques: Animal Model, Western Blot, Control, Software

Journal: Frontiers in Cardiovascular Medicine

Article Title: TGF-β-Containing Small Extracellular Vesicles From PM 2.5 -Activated Macrophages Induces Cardiotoxicity

doi: 10.3389/fcvm.2022.917719

Figure Lengend Snippet: TGF-β-containing sEV-induced production of collagen through TGF-β-Smad2/3 signaling pathway in cardiomyocytes. (A) Representative images of HL-1 cells that were incubated with the RAW264.7-sEV of PKH26-labeled sEV (red), cytoskeleton was stained with tubulin (green) and nuclei with DAPI (blue). Scale bar: 50 μm, 20 μm. (B) Relative mRNA expressions of α-SMA, mmp9, Col1α1, Col1α2, Col3α1, and TGF-β in HL-1 after being treated with RAW264.7-sEV analyzed by qRT-PCR. (C–G) Relative protein expressions of GF-β, α-SMA, Col I, and Col III in HL-1 were analyzed by Western blot. (H–J) Quantification of normalized p-Smad2/Smad2, p-Smad3/Smad3 in HL-1. All the data were presented as mean ± SEM ( t -test), n = 3–4, *indicates p < 0.05, **indicates p < 0.01.

Article Snippet: The cell samples were incubated overnight with anti-tubulin primary antibody at 4°C, followed by incubation with secondary antibody labeled with Alexa Fluor 488 (#ZF-0512, ZSGB-BIO, China) for 1 h at room temperature.

Techniques: Incubation, Labeling, Staining, Quantitative RT-PCR, Western Blot